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Proteintech arg 1
Arg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+arg+1/CD86+Antibody/pm41903309-120-54-61
Average 96 stars, based on 555 article reviews
arg 1 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Membrane:

Article Title: The mechanism of Vicenin-2 in ameliorating skin photoaging: involvement of m6A-modified macrophage polarization
Article Snippet: .. The membrane was blocked with blocking buffer for 1 h at room temperature, followed by an overnight incubation at 4 °C with the following primary antibodies: anti-KIAA1429 (30166-1-AP, 1:2000; Proteintech, China), anti-NF-κB p65 (10745-1-AP, 1:1000; Proteintech, China), anti- Phospho-NF-κB p65 (82335-1-RR, 1:2000; Proteintech, China), anti-β-actin (20536-1-AP, 1:4000; Proteintech, China), anti-iNOS (18985-1-AP, 1:5000; Proteintech, China), and anti-Arg-1 (16001-1-AP, 1:5000; Proteintech, China). .. After washing, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (LF102, 1:2000; Epizyme Biotech, China) for 1 h at room temperature in the dark.

Blocking Assay:

Article Title: The mechanism of Vicenin-2 in ameliorating skin photoaging: involvement of m6A-modified macrophage polarization
Article Snippet: .. The membrane was blocked with blocking buffer for 1 h at room temperature, followed by an overnight incubation at 4 °C with the following primary antibodies: anti-KIAA1429 (30166-1-AP, 1:2000; Proteintech, China), anti-NF-κB p65 (10745-1-AP, 1:1000; Proteintech, China), anti- Phospho-NF-κB p65 (82335-1-RR, 1:2000; Proteintech, China), anti-β-actin (20536-1-AP, 1:4000; Proteintech, China), anti-iNOS (18985-1-AP, 1:5000; Proteintech, China), and anti-Arg-1 (16001-1-AP, 1:5000; Proteintech, China). .. After washing, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (LF102, 1:2000; Epizyme Biotech, China) for 1 h at room temperature in the dark.

Article Title: Integrative network toxicology and single-cell transcriptomics reveal TP53 as a key mediator of PCBs-induced microglial dysfunction in diabetic retinopathy.
Article Snippet: .. After blocking with 5 % non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-p53 (1:1000, Cell Signaling Technology, #2524), antiiNOS (1:1000, Abcam, ab178945), anti-Arg-1 (1:1000, Proteintech, 66129–1-Ig), anti-Bax (1:1000, Abcam, ab32503), anti-Caspase-3 (1:1000, Cell Signaling Technology, #9662), anti-cleaved Caspase-3 (1:1000, Cell Signaling Technology, #9664), and anti-Bcl-2 (1:1000, Proteintech, 68103–1-Ig). β-actin (1:5000, Proteintech, 66009–1-Ig) was used as a loading control. ..

Article Title: TWEAK exacerbates allergic conjunctivitis via PKM2-dependent NF-κB activation and macrophage M1 polarization.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Incubation:

Article Title: The mechanism of Vicenin-2 in ameliorating skin photoaging: involvement of m6A-modified macrophage polarization
Article Snippet: .. The membrane was blocked with blocking buffer for 1 h at room temperature, followed by an overnight incubation at 4 °C with the following primary antibodies: anti-KIAA1429 (30166-1-AP, 1:2000; Proteintech, China), anti-NF-κB p65 (10745-1-AP, 1:1000; Proteintech, China), anti- Phospho-NF-κB p65 (82335-1-RR, 1:2000; Proteintech, China), anti-β-actin (20536-1-AP, 1:4000; Proteintech, China), anti-iNOS (18985-1-AP, 1:5000; Proteintech, China), and anti-Arg-1 (16001-1-AP, 1:5000; Proteintech, China). .. After washing, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (LF102, 1:2000; Epizyme Biotech, China) for 1 h at room temperature in the dark.

Article Title: Transglutaminase-2 Promotes Microglial Synaptic Phagocytosis and Ameliorates Epileptic Seizures by Inhibiting ABCA1 Ubiquitination.
Article Snippet: .. The sections were permeabilized with 0.3% Triton X- 100 at 37°C for 30 min, blocked with goat serum (Sevier, Wuhan, China) at 37°C for 1 h, and incubated with primary antibodies at 4°C for 16 h. The primary antibody panel included: anti- TGM2 (rabbit, Proteintech, cat. no. 15100- 1- AP, 1:100), anti–glial fibrillary acidic protein (GFAP; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–neuronal nuclei (NeuN; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–ionized calcium- binding adapter molecule (Iba- 1; rabbit, ZenBio, cat. no. 382207, 1:200), anti- PSD95 (rabbit, CST, cat. no. 3450S, 1:200), anti- CD68 (rabbit, Proteintech, cat. no. 28058- 1- AP, 1:100), anti- iNOS (rabbit, ZenBio, cat. no. 340668, 1:200), and anti- ARG- 1 (rabbit, Proteintech, cat. no. 66129- 1- AP, 1:800). .. After washing with PBS, the sections were incubated with a fluorescent secondary antibody (Aifang Bio, cat. no. AFIHC034; a universal, highly specific secondary antibody that has been specially engineered to simultaneously recognize primary antibodies from different species and enables multiplex labeling by coupling with different fluorescent dyes) at 37°C for 1 h in the dark.

Article Title: ​Treadmill running modulates infrapatellar fat pad remodeling in osteoarthritic rats in an intensity-dependent manner.
Article Snippet: .. The sections were then incubated overnight at 4°C with the following primary antibodies: anti-Arg-1 (16001-1-AP, 1:100, Proteintech), anti-iNOS (22226-1-AP, 1:800, Proteintech), anti-Col I (66761-1-IG, 1:500, Proteintech), and anti-α-SMA (14395-1-AP, 1:1000, Proteintech). ..

Article Title: Integrative network toxicology and single-cell transcriptomics reveal TP53 as a key mediator of PCBs-induced microglial dysfunction in diabetic retinopathy.
Article Snippet: .. After blocking with 5 % non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-p53 (1:1000, Cell Signaling Technology, #2524), antiiNOS (1:1000, Abcam, ab178945), anti-Arg-1 (1:1000, Proteintech, 66129–1-Ig), anti-Bax (1:1000, Abcam, ab32503), anti-Caspase-3 (1:1000, Cell Signaling Technology, #9662), anti-cleaved Caspase-3 (1:1000, Cell Signaling Technology, #9664), and anti-Bcl-2 (1:1000, Proteintech, 68103–1-Ig). β-actin (1:5000, Proteintech, 66009–1-Ig) was used as a loading control. ..

Article Title: TWEAK exacerbates allergic conjunctivitis via PKM2-dependent NF-κB activation and macrophage M1 polarization.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Binding Assay:

Article Title: Transglutaminase-2 Promotes Microglial Synaptic Phagocytosis and Ameliorates Epileptic Seizures by Inhibiting ABCA1 Ubiquitination.
Article Snippet: .. The sections were permeabilized with 0.3% Triton X- 100 at 37°C for 30 min, blocked with goat serum (Sevier, Wuhan, China) at 37°C for 1 h, and incubated with primary antibodies at 4°C for 16 h. The primary antibody panel included: anti- TGM2 (rabbit, Proteintech, cat. no. 15100- 1- AP, 1:100), anti–glial fibrillary acidic protein (GFAP; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–neuronal nuclei (NeuN; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–ionized calcium- binding adapter molecule (Iba- 1; rabbit, ZenBio, cat. no. 382207, 1:200), anti- PSD95 (rabbit, CST, cat. no. 3450S, 1:200), anti- CD68 (rabbit, Proteintech, cat. no. 28058- 1- AP, 1:100), anti- iNOS (rabbit, ZenBio, cat. no. 340668, 1:200), and anti- ARG- 1 (rabbit, Proteintech, cat. no. 66129- 1- AP, 1:800). .. After washing with PBS, the sections were incubated with a fluorescent secondary antibody (Aifang Bio, cat. no. AFIHC034; a universal, highly specific secondary antibody that has been specially engineered to simultaneously recognize primary antibodies from different species and enables multiplex labeling by coupling with different fluorescent dyes) at 37°C for 1 h in the dark.

CCK-8 Assay:

Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug‐Releasing Capability for the Programmable Repair of Spinal Cord Injury
Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit‐8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM‐HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti‐ iNOS (22226‐1‐AP, Proteintech, the US), anti‐Arg‐1 (66129‐1‐Ig, Proteintech, the US), and β‐actin (HRP‐60008) were purchased from Proteintech Group. ..

Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug-Releasing Capability for the Programmable Repair of Spinal Cord Injury.
Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit-8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM-HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti- iNOS (22226- 1-AP, Proteintech, the US), anti-Arg-1 (66129-1-Ig, Proteintech, the US), and β-actin (HRP-60008) were purchased from Proteintech Group. ..

Double Staining:

Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug‐Releasing Capability for the Programmable Repair of Spinal Cord Injury
Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit‐8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM‐HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti‐ iNOS (22226‐1‐AP, Proteintech, the US), anti‐Arg‐1 (66129‐1‐Ig, Proteintech, the US), and β‐actin (HRP‐60008) were purchased from Proteintech Group. ..

Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug-Releasing Capability for the Programmable Repair of Spinal Cord Injury.
Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit-8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM-HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti- iNOS (22226- 1-AP, Proteintech, the US), anti-Arg-1 (66129-1-Ig, Proteintech, the US), and β-actin (HRP-60008) were purchased from Proteintech Group. ..

RNA Extraction:

Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug‐Releasing Capability for the Programmable Repair of Spinal Cord Injury
Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit‐8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM‐HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti‐ iNOS (22226‐1‐AP, Proteintech, the US), anti‐Arg‐1 (66129‐1‐Ig, Proteintech, the US), and β‐actin (HRP‐60008) were purchased from Proteintech Group. ..

Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug-Releasing Capability for the Programmable Repair of Spinal Cord Injury.
Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit-8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM-HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti- iNOS (22226- 1-AP, Proteintech, the US), anti-Arg-1 (66129-1-Ig, Proteintech, the US), and β-actin (HRP-60008) were purchased from Proteintech Group. ..

Control:

Article Title: Integrative network toxicology and single-cell transcriptomics reveal TP53 as a key mediator of PCBs-induced microglial dysfunction in diabetic retinopathy.
Article Snippet: .. After blocking with 5 % non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-p53 (1:1000, Cell Signaling Technology, #2524), antiiNOS (1:1000, Abcam, ab178945), anti-Arg-1 (1:1000, Proteintech, 66129–1-Ig), anti-Bax (1:1000, Abcam, ab32503), anti-Caspase-3 (1:1000, Cell Signaling Technology, #9662), anti-cleaved Caspase-3 (1:1000, Cell Signaling Technology, #9664), and anti-Bcl-2 (1:1000, Proteintech, 68103–1-Ig). β-actin (1:5000, Proteintech, 66009–1-Ig) was used as a loading control. ..

Western Blot:

Article Title: M2 macrophage-mediated tigecycline nanoparticles for combating CRKP pneumonia via antibacterial and immunomodulatory therapy.
Article Snippet: Pneumonia caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) has a high mortality rate and exhibits multiple drug resistance, making current antibacterial treatments of limited efficacy.. To address the challenges of ineffective drug accumulation at the infection site and excessive inflammation leading to tissue damage, this study developed a cell-based nanobionic drug delivery system (denoted as PT@M2, PLGA-TIG nanoparticles loaded into M2 macrophages).. This system encapsulates poly(lactic-co-glycolic acid) (PLGA) loaded with tigecycline within M2-type macrophages, thereby achieving stable circulation, rapid release in acidic microenvironments, and targeted delivery to the lungs.



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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, <t>and</t> <t>Arg-1</t> expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, <t>and</t> <t>Arg-1</t> expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, <t>and</t> <t>Arg-1</t> expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, <t>and</t> <t>Arg-1</t> expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, <t>and</t> <t>Arg-1</t> expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, <t>and</t> <t>Arg-1</t> expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
Arg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers <t>(Arg‐1</t> and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).
Arginase 1 Arg 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, and Arg-1 expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

Journal: Cell Reports Medicine

Article Title: Light-driven thermotropic hydrogel with nanomodulator enables lipid intervention and cavity delivery for postsurgical tumor therapy

doi: 10.1016/j.xcrm.2026.102880

Figure Lengend Snippet: The effects of LA on the phenotypic characteristics of RAW264.7 cells and regulation of Ra on their phenotypic expression (A–C) The Kaplan-Meier survival curves of ABCA1, ABCG1, and PLIN4 in TCGA-SKCM. (D–J) The transcriptomic analysis of RAW264.7 cells with different treatments: (D) the volcano plots of LA group (culturing for three generations with LA) compared with control group (culturing for three generations without LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (E and F) GO and KEGG enrichment analysis of lipid synthesis/metabolism genes in LA group compared with control group; (G) the lipid synthesis/metabolism gene heatmap of control and LA groups; (H and I) GO and KEGG enrichment analysis of immune-related genes in LA group compared with control group; (J) the immune-related gene heatmap of control and LA groups ( n = 3). (K and L) The flow cytometry assay of LD levels in CD206 − RAW264.7 and CD206 + RAW264.7 ( n = 3). (M) The strategy of lipid droplet metabolism to regulate RAW264.7 phenotype. (N and O) The flow cytometry assay of LD levels in RAW264.7 treated with different Ra concentrations ( n = 3). (P and Q) Perilipin 2, iNOS, and Arg-1 expression levels of RAW264.7 treated with Ra ( n = 3). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (L), (N), and (Q); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

Article Snippet: Anti-mouse Arg-1 Antibody , Abclonal , Cat#A4923; RRID:AB_2863390.

Techniques: Expressing, Control, Flow Cytometry

The modulation of R@LOX on the polarization of macrophages (A) Representative flow cytometry dot plots. (B) The proportion of M1/M2 in BMDMs ( n = 3). (C–F) The relative mRNA expression of (C) CD86, (D) NOS2, (E) CD206, and (F) Arg-1 in BMDMs ( n = 3). (G–K) The transcriptomic analysis results of RAW264.7 cells: (G) the volcano plots of R@LOX group (culturing with R@LOX and LA) compared with control group (culturing with only LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (H and I) GO and KEGG enrichment analysis of SDEGs in R@LOX group compared with control group; (J and K) the heatmap of control and R@LOX group genes. Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (C), (D), (E), and (F) and unpaired two-tailed Student’s t test for (B). n.s., not significant; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. See also .

Journal: Cell Reports Medicine

Article Title: Light-driven thermotropic hydrogel with nanomodulator enables lipid intervention and cavity delivery for postsurgical tumor therapy

doi: 10.1016/j.xcrm.2026.102880

Figure Lengend Snippet: The modulation of R@LOX on the polarization of macrophages (A) Representative flow cytometry dot plots. (B) The proportion of M1/M2 in BMDMs ( n = 3). (C–F) The relative mRNA expression of (C) CD86, (D) NOS2, (E) CD206, and (F) Arg-1 in BMDMs ( n = 3). (G–K) The transcriptomic analysis results of RAW264.7 cells: (G) the volcano plots of R@LOX group (culturing with R@LOX and LA) compared with control group (culturing with only LA), and both the blue and red dots represented SDEGs (|log 2 FC|≥1 and q < 0.05); (H and I) GO and KEGG enrichment analysis of SDEGs in R@LOX group compared with control group; (J and K) the heatmap of control and R@LOX group genes. Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (C), (D), (E), and (F) and unpaired two-tailed Student’s t test for (B). n.s., not significant; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. See also .

Article Snippet: Anti-mouse Arg-1 Antibody , Abclonal , Cat#A4923; RRID:AB_2863390.

Techniques: Flow Cytometry, Expressing, Control, Two Tailed Test

In vivo immune activation mechanism of LTG@RL against postsurgical melanoma recurrence (A–D) Relative mRNA expressions of (A) TNF-α, (B) IFN-γ, (C) Arg-1, (D) NOS2 in tumor tissues ( n = 3). (E–S) Flow cytometric analysis of (E and F) T cells, (G and H) CD8 + T cells, (I and J) CD86 + TAM, (K and L) CD86 + NOS2 + TAM, (M and N) CD206 + TAM, (O and P) CD206 + Arg-1 + TAM, proportion of (Q) M1/M2 and (R and S) PD-L2 + TAM in tumors ( n = 3, the unstained control, compensation control, and viability dye control were employed). (T) Flow cytometry assay of LD levels in CD11b + F4/80 + cells in tumors ( n = 5). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (A), (B), (C), (D), and (T), and unpaired two-tailed Student’s t test for (F), (H), (J), (L), (N), (P), (Q), and (S); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

Journal: Cell Reports Medicine

Article Title: Light-driven thermotropic hydrogel with nanomodulator enables lipid intervention and cavity delivery for postsurgical tumor therapy

doi: 10.1016/j.xcrm.2026.102880

Figure Lengend Snippet: In vivo immune activation mechanism of LTG@RL against postsurgical melanoma recurrence (A–D) Relative mRNA expressions of (A) TNF-α, (B) IFN-γ, (C) Arg-1, (D) NOS2 in tumor tissues ( n = 3). (E–S) Flow cytometric analysis of (E and F) T cells, (G and H) CD8 + T cells, (I and J) CD86 + TAM, (K and L) CD86 + NOS2 + TAM, (M and N) CD206 + TAM, (O and P) CD206 + Arg-1 + TAM, proportion of (Q) M1/M2 and (R and S) PD-L2 + TAM in tumors ( n = 3, the unstained control, compensation control, and viability dye control were employed). (T) Flow cytometry assay of LD levels in CD11b + F4/80 + cells in tumors ( n = 5). Data are represented as mean ± SD. p values were determined by one-way ANOVA with Tukey’s test for (A), (B), (C), (D), and (T), and unpaired two-tailed Student’s t test for (F), (H), (J), (L), (N), (P), (Q), and (S); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

Article Snippet: Anti-mouse Arg-1 Antibody , Abclonal , Cat#A4923; RRID:AB_2863390.

Techniques: In Vivo, Activation Assay, Control, Flow Cytometry, Two Tailed Test

Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).

Journal: Clinical and Experimental Pharmacology & Physiology

Article Title: Mechanisms of IL‐17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

doi: 10.1111/1440-1681.70116

Figure Lengend Snippet: Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).

Article Snippet: Sections were then incubated overnight at 4°C with primary antibodies against: E‐cadherin (Boster, China), Collagen III (Boster, China), inducible nitric oxide synthase (iNOS) (Boster, China), CD86 (Boster, China), arginase‐1 (Arg‐1) (Boster, China), CD163 (Boster, China), α‐smooth muscle actin (α‐SMA) (Cell Signalling Technology, USA).

Techniques: Immunohistochemistry, Western Blot, Expressing