arg 1 (Proteintech)
Structured Review
Arg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+arg+1/CD86+Antibody/pm41903309-120-54-61
Average 96 stars, based on 555 article reviews
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Membrane:Article Title: The mechanism of Vicenin-2 in ameliorating skin photoaging: involvement of m6A-modified macrophage polarization Article Snippet: .. The membrane was blocked with blocking buffer for 1 h at room temperature, followed by an overnight incubation at 4 °C with the following primary antibodies: anti-KIAA1429 (30166-1-AP, 1:2000; Proteintech, China), anti-NF-κB p65 (10745-1-AP, 1:1000; Proteintech, China), anti- Phospho-NF-κB p65 (82335-1-RR, 1:2000; Proteintech, China), anti-β-actin (20536-1-AP, 1:4000; Proteintech, China), anti-iNOS (18985-1-AP, 1:5000; Proteintech, China), and Blocking Assay:Article Title: The mechanism of Vicenin-2 in ameliorating skin photoaging: involvement of m6A-modified macrophage polarization Article Snippet: .. The membrane was blocked with blocking buffer for 1 h at room temperature, followed by an overnight incubation at 4 °C with the following primary antibodies: anti-KIAA1429 (30166-1-AP, 1:2000; Proteintech, China), anti-NF-κB p65 (10745-1-AP, 1:1000; Proteintech, China), anti- Phospho-NF-κB p65 (82335-1-RR, 1:2000; Proteintech, China), anti-β-actin (20536-1-AP, 1:4000; Proteintech, China), anti-iNOS (18985-1-AP, 1:5000; Proteintech, China), and Article Title: Integrative network toxicology and single-cell transcriptomics reveal TP53 as a key mediator of PCBs-induced microglial dysfunction in diabetic retinopathy. Article Snippet: .. After blocking with 5 % non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-p53 (1:1000, Cell Signaling Technology, #2524), antiiNOS (1:1000, Abcam, ab178945), Article Title: TWEAK exacerbates allergic conjunctivitis via PKM2-dependent NF-κB activation and macrophage M1 polarization. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Incubation:Article Title: The mechanism of Vicenin-2 in ameliorating skin photoaging: involvement of m6A-modified macrophage polarization Article Snippet: .. The membrane was blocked with blocking buffer for 1 h at room temperature, followed by an overnight incubation at 4 °C with the following primary antibodies: anti-KIAA1429 (30166-1-AP, 1:2000; Proteintech, China), anti-NF-κB p65 (10745-1-AP, 1:1000; Proteintech, China), anti- Phospho-NF-κB p65 (82335-1-RR, 1:2000; Proteintech, China), anti-β-actin (20536-1-AP, 1:4000; Proteintech, China), anti-iNOS (18985-1-AP, 1:5000; Proteintech, China), and Article Title: Transglutaminase-2 Promotes Microglial Synaptic Phagocytosis and Ameliorates Epileptic Seizures by Inhibiting ABCA1 Ubiquitination. Article Snippet: .. The sections were permeabilized with 0.3% Triton X- 100 at 37°C for 30 min, blocked with goat serum (Sevier, Wuhan, China) at 37°C for 1 h, and incubated with primary antibodies at 4°C for 16 h. The primary antibody panel included: anti- TGM2 (rabbit, Proteintech, cat. no. 15100- 1- AP, 1:100), anti–glial fibrillary acidic protein (GFAP; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–neuronal nuclei (NeuN; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–ionized calcium- binding adapter molecule (Iba- 1; rabbit, ZenBio, cat. no. 382207, 1:200), anti- PSD95 (rabbit, CST, cat. no. 3450S, 1:200), anti- CD68 (rabbit, Proteintech, cat. no. 28058- 1- AP, 1:100), anti- iNOS (rabbit, ZenBio, cat. no. 340668, 1:200), and Article Title: Treadmill running modulates infrapatellar fat pad remodeling in osteoarthritic rats in an intensity-dependent manner. Article Snippet: .. The sections were then incubated overnight at 4°C with the following primary antibodies: Article Title: Integrative network toxicology and single-cell transcriptomics reveal TP53 as a key mediator of PCBs-induced microglial dysfunction in diabetic retinopathy. Article Snippet: .. After blocking with 5 % non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-p53 (1:1000, Cell Signaling Technology, #2524), antiiNOS (1:1000, Abcam, ab178945), Article Title: TWEAK exacerbates allergic conjunctivitis via PKM2-dependent NF-κB activation and macrophage M1 polarization. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Binding Assay:Article Title: Transglutaminase-2 Promotes Microglial Synaptic Phagocytosis and Ameliorates Epileptic Seizures by Inhibiting ABCA1 Ubiquitination. Article Snippet: .. The sections were permeabilized with 0.3% Triton X- 100 at 37°C for 30 min, blocked with goat serum (Sevier, Wuhan, China) at 37°C for 1 h, and incubated with primary antibodies at 4°C for 16 h. The primary antibody panel included: anti- TGM2 (rabbit, Proteintech, cat. no. 15100- 1- AP, 1:100), anti–glial fibrillary acidic protein (GFAP; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–neuronal nuclei (NeuN; rabbit, Proteintech, cat. no. 16825- 1- AP, 1:100), anti–ionized calcium- binding adapter molecule (Iba- 1; rabbit, ZenBio, cat. no. 382207, 1:200), anti- PSD95 (rabbit, CST, cat. no. 3450S, 1:200), anti- CD68 (rabbit, Proteintech, cat. no. 28058- 1- AP, 1:100), anti- iNOS (rabbit, ZenBio, cat. no. 340668, 1:200), and CCK-8 Assay:Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug‐Releasing Capability for the Programmable Repair of Spinal Cord Injury Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit‐8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM‐HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti‐ iNOS (22226‐1‐AP, Proteintech, the US), Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug-Releasing Capability for the Programmable Repair of Spinal Cord Injury. Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit-8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM-HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti- iNOS (22226- 1-AP, Proteintech, the US), Double Staining:Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug‐Releasing Capability for the Programmable Repair of Spinal Cord Injury Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit‐8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM‐HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti‐ iNOS (22226‐1‐AP, Proteintech, the US), Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug-Releasing Capability for the Programmable Repair of Spinal Cord Injury. Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit-8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM-HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti- iNOS (22226- 1-AP, Proteintech, the US), RNA Extraction:Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug‐Releasing Capability for the Programmable Repair of Spinal Cord Injury Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit‐8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM‐HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti‐ iNOS (22226‐1‐AP, Proteintech, the US), Article Title: Nanogel Integrated Zwitterionic Injectable Hydrogel with Sequential Drug-Releasing Capability for the Programmable Repair of Spinal Cord Injury. Article Snippet: BCA kit, RIPA lysis buffer, protease inhibitor and phosphatase inhibitor were purchased from Shanghai Beyotime Biotechnology Co. .. Cell counting kit-8 (CCK8), Calcein AM/PI Live/Dead cell double staining kit and RNA extraction kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. RMPI 1640 and DMEM-HG were purchased from Thermo Fisher Scientific Inc. PrimeScriptTM RT reagent kit was purchased from Takara Biomedical Technology (Beijing) Co., Ltd. Anti- iNOS (22226- 1-AP, Proteintech, the US), Control:Article Title: Integrative network toxicology and single-cell transcriptomics reveal TP53 as a key mediator of PCBs-induced microglial dysfunction in diabetic retinopathy. Article Snippet: .. After blocking with 5 % non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-p53 (1:1000, Cell Signaling Technology, #2524), antiiNOS (1:1000, Abcam, ab178945), Western Blot:Article Title: M2 macrophage-mediated tigecycline nanoparticles for combating CRKP pneumonia via antibacterial and immunomodulatory therapy. Article Snippet: Pneumonia caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) has a high mortality rate and exhibits multiple drug resistance, making current antibacterial treatments of limited efficacy.. To address the challenges of ineffective drug accumulation at the infection site and excessive inflammation leading to tissue damage, this study developed a cell-based nanobionic drug delivery system (denoted as PT@M2, PLGA-TIG nanoparticles loaded into M2 macrophages).. This system encapsulates poly(lactic-co-glycolic acid) (PLGA) loaded with tigecycline within M2-type macrophages, thereby achieving stable circulation, rapid release in acidic microenvironments, and targeted delivery to the lungs. |

